SOCHOR Jiří, SALAŠ Petr, ZEHNÁLEK Josef, KRŠKA Boris, ADAM Vojtěch, HAVEL Ladislav, KIZEK René
(Mendelova univerzita v Brně)
Protokol pro fotometrické stanovení antioxidační aktivity v biologickém vzorku An assay for spectrometric determination of antioxidant activity of a biological extract
Antioxidant activity is defined as the ability of a compound or a mixture of compounds inhibit oxidative degradation
of various substances (e.g., preventing lipid peroxidation). Methods used for determination of antioxidant activity bases
on the direct reaction of compound of interest with radicals (scavenging or complexing) or on the reaction with transition
metals. The aim of this study was to optimize, automate and accurately describe the protocols of five photometric methods:
the DPPH, TEAC, FRAP, DMPD and Free Radicals Kit, used to determine the antioxidant activity of a biological sample. Methods
were standardized using the substance Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid). Advantages, disadvantages
and limitations of each method were discussed.